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Western blot quantification imagej4/11/2024 ![]() Press Ctrl and 1 to set first lane (Command and 1 on the Mac).Ĭlick the centre of the square and drag it across to the next lane. Use the square selection tool to highlight the first lane. Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S.Convert the image to 8-bit using ImageJ function (Image→Type→8-bit). You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not: Export-Re-export Requirements and Enhanced Proliferation Control Initiative.Įxport control laws, including but not limited to the U.S. use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria) or.sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria) or.transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S.transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses or. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).Īll rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.įor more information regarding U.S. Department of the Treasury, Office of Foreign Assets Control.Housekeeping Protein Normalization Protocol Introduction export laws and regulations, contact the U.S. In quantitative Western blotting (QWB), normalization mathematically corrects for unavoidable sample-to-sample and lane-to-lane variation by comparing the target protein to an internal loading control. Using a Housekeeping Protein (HKP) as an Internal Loading Control The internal loading control is used as an indicator of sample protein loading, to correct for loading variation and confirm that observed changes represent actual differences between samples.įor more normalization related resources, see " Further Reading". Housekeeping proteins (HKPs) are routinely used as loading controls for Western blot normalization. Accurate normalization requires stable expression of the HKP across all experimental conditions and treatments. Because HKP normalization relies on a single indicator of sample loading, variation in HKP expression leads to inconsistent estimation of sample loading and introduces experimental error that may alter data analysis.įor widely-used HKPs (such as actin, tubulin, and GAPDH), stable expression has generally been assumed. However, expression of common HKPs is now known to vary in response to certain experimental conditions, including cell confluence, disease state, drug treatment, and cell or tissue type.īefore an HKP is used for Western blot normalization, stable expression must be validated for the specific experimental context and treatments. ![]() For more information, see the Housekeeping Protein Validation Protocol ( /HKP-Validation LI-COR). This protocol describes how to use a housekeeping protein for Western blot normalization and quantitative analysis. This protocol is intended for use with near-infrared fluorescent Western blots. Saturated bands and sample overloading frequently compromise the accuracy of QWB. ![]()
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Get fork onto github desktop4/11/2024 ![]() ![]() The next screen will show a link to the GitHub Desktop web page, and you should download and install the application.įigure 10.27: To clean up your code editor workspace, right-click to Remove Project Folder. Click the Code > Open with GitHub Desktop drop-down menu button near the middle of your screen, as shown in Figure 10.18. In your browser, navigate to, using your GitHub username, to access the repo you created in the Copy, Edit, and Host a Simple Leaflet Map Template section of this chapter. Go to the GitHub web repo you wish to copy to your local computer.Let’s use GitHub Desktop to pull a copy of your leaflet-map-simple template to your local computer, make some edits in a code editor, and push your commits back up to GitHub. ![]() Tip: Currently, GitHub Desktop is not supported for Chromebooks, but Chrome’s Web Store offers several text editors, such as Text and Caret, which offer some of the functionality described below. Also, tools designed primarily as code editors, such as Pulsar, will work better than plain-text editors bundled with operating systems, such as TextEdit for Mac or NotePad for Windows. Tip: Word-processor tools such as Microsoft Word are not good choices for code editors. Any text editor allows you to view and edit code repos on your local computer more easily than the GitHub web interface. When you connect your GitHub web account to GitHub Desktop, it allows you to “pull” the most recent version of the code to your local computer’s hard drive, make and test your edits, and “push” your commits back to your GitHub web account. To speed up your work on Mac or Windows, we recommend that you download the free GitHub Desktop tool, plus any code editor, such as new favorite open-source option, Pulsar, based on the former Atom editor tool, but there are other options. But the web interface will feel very slow if you edit or upload multiple files in your repo. GitHub Desktop and Code Editor to Work EfficientlyĮditing your code through the GitHub web interface is a good way to start, especially if you only need to make a few edits or upload a couple of files to your repo. Zotero and Better BibTeX for Notes and Biblio.Style Guide for Hands-On Data Visualization.GitHub Desktop and Code Editor to Work Efficiently.Create a New Repo and Upload Files on GitHub.Copy, Edit, and Host a Simple Leaflet Map Template.Our Open-Access Web Edition: Why and How. ![]()
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![]() ![]() Wave’s easy-to-read dashboard shows you your most important financial info at a glance. ![]() Automatically generated journal entries.Crucial accounting reports and general ledger.Easy, customizable income and expense tracking.Unlimited bank account and credit card connections.Automatic bank account and credit card syncing for accurate, up-to-date financial records. ![]() It’s one of our top accounting software picks for small-business owners, and for good reason. But if you're fine with basic invoicing and transactions, it's honestly hard to beat Zoho Invoice.Įven if Wave weren’t completely free, we’d still be blown away by how much it offers. Otherwise, pricing for Zoho Books starts at $15 a month, which makes it a solid alternative to much pricier accounting solutions like QuickBooks Online. If that describes your business, we highly recommend adding Zoho Books to your Zoho Invoice plan. If you want more extensive accounting features, Zoho Books has a free plan for businesses with under $50K USD in annual revenue. With a stellar (and again, free) bookkeeping and invoicing app, it's also good for freelancers on the go. Thanks to its its built-in time tracking (which syncs easily with invoices), estimate-to-invoice conversion, and client portal for straightforward collaboration, Zoho Invoice is ideal for freelancers who frequently collaborate with customers.
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Create online signature for pdf4/11/2024 ![]() ![]() The signed contract in PDF can be downloaded through the email automatically sent by Oneflow after it’s signed by all parties.Sign the contract, and voilà! You’ve just signed a PDF document online with a legally binding electronic signature.When your counterparty receives, opens, or signs the PDF contract you’ve just sent via Oneflow, you will automatically get a notification.You will be able to write a personal message or add a personal video to the contract directly in Oneflow.Add the counterparty’s email address on the right panel, and your contract is ready to send!.Upload the PDF document you want to sign to the template.Choose “create new contract” and choose the “PDF template”.Now it’s time for you to get that PDF document signed. When you click on the link from the Oneflow email, you will have to complete your registration by filling in the required fields.This is to make sure that no one else is signing you up without your permission. Once you’ve created your free account, you’ll get an email from Oneflow to verify your email address.Alternatively, you can create a free account in Oneflow here. ![]() Choose one of the valid electronic signature providers here.Add an explanatory name to the PDF document you want to sign and save it in your local drive.Simply create the contract you intend to sign and export it as PDF. First of all, you create your document in PDF you want to sign from any document editor such as Google Docs or Microsoft Office Word.Here’s a step-by-step guide to how to achieve that: That’s why we’ve listed a number of viable alternatives to electronic signature providers here. However, many first-time users of PDF online signing might be cautious of uploading sensitive documents online which contain personal information, such as contracts. Signing PDF documents online is pretty straightforward. Simply google “sign a pdf online” and you’ll get more than a quarter of a billion hits. It doesn’t matter if it is a consulting agreement, a freelance contract, a power of attorney, or minutes of a board meeting, you can easily sign a PDF online with any of the many services that are available today. Do you want to sign a PDF online that requires a legally binding digital or electronic signature? ![]()
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Emcee wedding script4/11/2024 ![]() I think I’ve had the good fortune to meet most of you here today at some point or another, but for those of you whom I haven’t met, my name is. I know how deeply these two care for and love one another, and I feel privileged to be here today among all of you as a witness of their commitment to a lifetime of love for one another. But about X years ago s/he met NAME, who is pretty wonderful, too. For NAME, that happened about 20 years ago when s/he met me and we became best friends. The person with whom you share a bond so special that it transcends normal relationships and becomes something so pure and so wonderful, that you can’t imagine spending another day of your life without them. The person who makes the world a beautiful and magical place. The most remarkable moment in life is when you meet the person who makes you feel complete. I truly can’t think of a better venue than _ for an occasion that I know is not only monumental for the wedded couple to be but for all of us who are lucky to know and love them as individuals but even more so as a perfect pairing. It’s no accident that each of you is here today, and each of you was invited to be here because you represent someone important in the individual and collective lives of NAME and NAME. What do you guys think of these ideas? Tacky? haha again it depends on the crowd but I have seen them done before and they were pretty funny.Show more Funny Marriage Ceremony Script For Traditional Weddingįirst, I’d like to begin by welcoming everyone and thanking every one of you for being here on this happiest of days. say that their keys were in the passenger side of the car with the car lights on. This is a good way to get all guests to know more about each person as some guests will not know the groom well and some will not know the bride.Īnother is to describe a car with its lights on in the parking lot, making up a ridiculous description of what is in the car. Then ask the couple the answers to the questions and make up funny alternatives. Make 10-15 MC choice questions about the couple like where they went on their first date, etc. ![]() Quiz called “How well do you know bride and groom”. Write a quiz before hand and put copies on each table, or at each place setting. Then saying……if any guys have a key to the groom’s house… I suppose now would also be the time to give it back” – then have a guy return his key… – Give out keys to several girls in the audience before hand and say something like “now that X is getting married, any girls that have one of his house keys, I think now’s the time to turn it in” – then have a long line of girls coming up to return the keys and put them in a dish at the head table. what do you bees think of this? Of course it entirely depends on the crowd. Here are some funny things that I have seen at previous weddings. We are lucky we won’t be MC’ing alone! Good luck to you too □ My co-MC will say how he knows a groom and we might throw in a funny story. I am not planning to be a completely phoney the whole evening, just was looking for some inspiration for ideas to incorporate a few jokes. also, I do recognize the best jokes are personal stories that are about the bride and groom.I also plan to write an introduction, to welcome guests and briefly state how I know the bride. I was looking for advice regarding funny things that people have seen other people do at previous weddings, not advice that I should try to avoid being funny. Wedding: August – Thank you for not making me feel like a total loser! haha.You are not alone in your google search either lol: ![]() I think if I have my hair and makeup rocking that will help. I’m hoping he will have some stories too.Īside from that, I like what a pp said about being confident and trying to get peoples names right. ![]() I have a co-mc, so I’ll also be touching base with him to see what he thinks… Someone I know and is pretty affable and nice. I’m also going to think of some small story of the couple to keep in my pocket in case the jokes don’t seem to fit the mood. I think telling people my/your (good) first impressions of them would be a good way to go. I met both separatly so that should help kill some time if I need to do so. Going to write an intro, saying how I know the bride and groom. It’s something I’ve traded back and forth with the bride and also a normal thing for me, so hopefully they don’t fall too flat. I’m going to google some silly/lame jokes and have a sheet of them to handle any dead spaces. Dude, I’m in the same boat! Bumping to follow along, and also to share my thoughts so far. ![]()
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Budget planner bi weekly with calendar4/11/2024 ![]() Budgeting takes time you must review bills to see where money is going.It can be challenging for irregular income like when you get paid biweekly. ![]() Budgeting requires frequent monitoring and adjustments. ![]() Helps save by allocating a portion of each paycheck.Easier cash flow management for bills and expenses.Matches well with biweekly pay schedules.More frequent budgeting keeps you engaged with your finances.Free templates are available to help you budget for $0- like mine!.This process helps you break free from the paycheck-to-paycheck cycle, plan for bills, make room for discretionary spending, and save for future goals. Now that you’ve collected all your data let’s set up your biweekly budget.Ī biweekly budget ensures you have enough funds to cover expenses for each two weeks.īy organizing and tracking your income and expenses this way, you clearly understand your financial spending every two weeks. With your goals in mind, allocate a specific amount from each check to savings and investments. Stay on track to build a strong financial cushion for the future. I like realistic and specific goals, writing them down and visualizing them on a vision board. Setting financial goals is crucial for a productive biweekly budget. Decide On Your Savings, Investing, & Financial Goals This step ensures you can cover expenses even during months with a tighter budget. If your earnings vary every month, create a budget based on your smallest paycheck. This is crucial if you get paid biweekly- make sure those numbers are right! When reviewing your checks, track your final pay. Now, let’s determine your actual earnings! Collect your pay stubs and other income sources. Here’s a helpful tip: Be honest about your spending! Estimate the amount you’ll spend on each and assign them to the appropriate paycheck.Ĭreate a list of fixed bills (rent, mortgage, utilities, insurance) and variable expenses (groceries, transportation, entertainment).ĭon’t forget to add due dates so you know when your bills overlap with your paychecks or when multiple bills hit at once and you need to save extra to cover things. To prepare your biweekly budget, gather your bills and identify your expenses to know where your money goes every week. Then hit print and get ready to fill it out. The templates will download as a PDF, and you can download it with the “arrow” button. Download & PrintĪfter opening the biweekly budget, go ahead and download and print it. Simply click on the image above to access it! 2. Here’s my biweekly budget template for you. I’ve used this biweekly system to watch what I spend, pay off debt, and set a nice little nest egg aside, so I’ll walk you through the steps to use your biweekly budget template, too! 1. If you want to start biweekly budgeting, just follow the steps below to download our user-friendly biweekly budget template. Using a Biweekly Budget : Simplify the process with my handy template and budget your way to better finances.īiweekly Budget Template: 6 Easy Steps To FollowĪ biweekly budget planner is easy to use, especially if you get paid biweekly, because it aligns nicely with your pay cycle.Creating a Savings Plan : Allocate a specific amount from each paycheck towards savings to build a financial cushion. ![]()
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What it feels like to have scid4/11/2024 ![]() “We launched the mobile Virtual Dementia Tour about a year after and, within two months, I had to buy my second because it was so successful. This gave us the opportunity to control the environment, protect our delegates, and ensure that the experience they got was fulfilling and targeted in the right way. However, due to logistical issues, Knight and the team had to adapt the experience to meet demand and ensure that it was as effective as possible. “Most people would have given up within six months, but I persevered because I knew it was the right thing to do,” Knight continued.Īfter months of knockbacks, the company eventually started receiving orders to deliver the Virtual Dementia Tour in care homes. What I realised is that our industry is slow to change. I kept getting told it would never work in this country and that it was not realistic. The initial response I received was very negative. “I spent a year travelling around the UK, showing people the Virtual Dementia Tour. Although some organisations, such as Stirling University, had briefly experimented with the technology, it was not yet widely used within the UK. It was quite remarkable,” Knight added.Įager to learn more about the experience, Knight contacted the company behind the innovation. The person put the equipment on and then started to exhibit identical behaviours and noises to their mum. “I watched some videos and one stuck with me of a family member whose mum had dementia. I went on a mission to find something that we could adopt or produce ourselves and it became really obvious that there wasn’t much on the market and that AI was still a little bit behind.” A new training experienceĪfter researching extensively online, Knight discovered a concept called the Virtual Dementia Tour, owned by a company called Second Wind Dreams in America and developed by dementia specialist P K Beville. Practical training is amazing, but I started to look at technology to see if I could use that to improve the impact of training for people. “However, there was always a niggle with me. Not only is it theoretical, everything is practical. We made a commitment to invest in practical training, so we purchased our own hoists, slings, and slide sheets. “The downside with most training providers is that a lot of them will use equipment already provided by the care industry. Glenn Knight, Managing Director of Training 2 Care, explained: “About four or five years in, we realised that we did not really have a USP and were doing pretty much the same as what everyone else was doing. After several years of working solely within this focus, the company made the decision to expand its offering. Launched in early 2010, Training 2 Care first formed as a mandatory training provider for the care sector. When it comes to professional training, admiral nurses, specially trained in dementia, are becoming a common feature in hospitals and care homes, and autism specialists are frequently utilised in establishments such as schools and community services.Įven with resources like this, however, how far can our understanding stretch if we do not personally experience living with a syndrome or disorder like autism or dementia? One organisation challenging this question is Training 2 Care, which delivers experiential training designed to provide people with the closest possible representation of what it actually feels like to have dementia or autism. Managing Director Glenn Knight spoke to Health Europa to explain more.įrom charities and organisations to celebrities and social media influencers, many people are continually working to increase awareness and understanding of syndromes and disorders such as dementia and autism. Alcohol brain fog: How to start healing your brain.© Daniel Jones Training 2 Care’s unique experiential training provides users with a glimpse of what it is like to live as a person with dementia or autism. MIND and Mediterranean diets linked to fewer signs of Alzheimer’s brain pathology. What is COVID-19 brain fog-and how can you clear it? Common anticholinergic drugs like Benadryl linked to increased dementia risk. Scientific insights into brain fog during the menopausal transition. ![]() ![]() Stuck in a brain fog? Look in your medicine cabinet. Puzzling symptoms: Eating disorders and the brain. Spilling the beans: How much caffeine is too much?Ĭedars-Sinai. The effects of vitamin D-enriched mushrooms and vitamin D3 on cognitive performance and mood in healthy elderly adults: A randomised, double-blinded, placebo-controlled trial. ![]() ![]() Zajac IT, Barnes M, Cavuoto P, Wittert G, Noakes M. Vitamins and minerals for energy, fatigue and cognition: A narrative review of the biochemical and clinical evidence. Tardy AL, Pouteau E, Marquez D, Yilmaz C, Scholey A. Here’s why you’re dealing with brain fog-and what you can do to fight it. ![]()
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Color wheel picker html4/11/2024 ![]() ![]() They are primarily used by web designers, graphic designers, computer programmers, and digital illustrators.Ĭhoosing the correct web colors can be exhausting, but it is a great skill to have, especially for marketing purposes. HTML color codes are used within HTML and CSS to create web design color schemes. To use a selected color code within your web page, you could place the following attribute within a given element to change its background color to red: style="background-color: #ff0000 " What are HTML color codes used for? To pick RGB 0-1 colors, check out our RGB 0-1 Color Picker. RGB 0-1 or Float RGB is another alternative representation of RGB colors that uses three decimal numbers between 0 and 1 to represent the color: one each for red, green, and blue, and one for an optional alpha channel. A color chart provides a listing of common colors for quick selection. To pick RGB 565 colors, check out our RGB 565 Color Picker. Pick a color: You can use the input type'color' attribute to create a color picker: Example. Online color picker to view shades, tints, color codes, and complementary colors. ![]() You can choose from a color chart, a color picker or a color palette to find the perfect color combination for your website. Finding the perfect color has never been easier with our user-friendly color picker tool. A color picker allows a user to select aĬolor by clicking on visual range of color to pin-point an exact code. HTML Color Codes HTML Color Chart is a website that provides you with HTML color codes for your website design. Common color codes are in the forms of:Ī keyword name, a hexadecimal value, a RGB (red, green, blue) triplet, or a HSL (hue, saturation, lightness) triplet.ĭifferent values allow for 16,777,216 potential colors to be chosen.įor example, the color red can be identifier using the following formats:īecause there are so many colors to choose from, tools have been created to make the task of selection much simpler. Well organized and easy to understand Web building tutorials with lots of examples of how to use HTML, CSS, JavaScript, SQL, Python, PHP, Bootstrap, Java, XML and more. Visually choose colors from all the possible combinations and get the Hex color code, RGB color codes and also the HSL codes. A HTML color code is an identifier used to represent a color on the web and within other digital assets. With this tool youll be able to select from 16,777,216 colors. ![]()
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Calculate velocity using a pitot tube4/10/2024 ![]() If a second piezometric opening records the static pressure in the undisturbed flow (P 0), the velocity of the flow may be inferred from the pressure difference (P s-P 0). In this case the tapping point, typically connected to some form of manometer, provides a direct measure of the stagnation pressure, or total pressure, P s. A hydrant’s inside diameter is usually 4. Measure the inside diameter of the outlet nozzle or hydrant outlet where flow occurs. Assume the coefficient of the pitot tube as 0.98. If the density of air and water are 1.13 kg/m kg/m3, respectively, determine the velocity of air. The manometer shows a difference in head of 5 cm of water. ![]() ![]() An annubar consists of several pitot tubes placed across a pipe to provide an approximation of the velocity. The flow rate can be determined from the difference between the static and dynamic pressures, which is the velocity head of the fluid flow. Slowly release the main valve until air is vented. A pitot static tube is used to measure the velocity of air flowing through a duct. The pitot tube measures the static and dynamic pressures of the fluid at one point in the pipe. ![]() If this is aligned with its central axis in the direction of the flow ( Figure 1a) the fluid will accelerate around the upstream face with minimal energy losses, and a stagnation point arises at the piezometric opening. In this video we demonstrate how to use a pitot tube and a manometer to measure velocity pressure, air velocity and airflow. Flush the residual hydrant to remove any sediment and attach a nozzle cap with a gauge to the hydrant’s outlet. In its simplest form this instrument consists of a symmetrical body such as a cylinder, cone, or hemisphere with a small hole or piezometric opening drilled along its central axis. If circumstances do not permit or require an accurate traverse, center the pitot tube in the duct, determine the pressure differential (velocity pressure), calculate actual center velocity, and multiply this value by 0.9. The total pressure tube, or Pitot tube, provides a common method of measuring the stagnation pressure within a pipe, channel or duct flow. Using velocity pressures recorded at each location, calculate velocities and average them for final velocity value. ![]()
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Rome total war 2 best factions4/10/2024 ![]() The patrician Scipii family is devoted to one cause: grinding those Carthaginian scum into submission, and if that is not possible, into the desert sands. This would mean the pointless upkeep of armies that need to be out in the field. Another problem is that, to pacify captured regions, the player will have to garrison their armies in the city, where they can be retrained, but they would not see much action. Extreme caution is required (use spies and assassins). ![]() One problem with this is that the dark forests beyond Roman lands can provide perfect platforms for ambushes and the like. If the player so chooses, they can blitzkrieg through the barbarian lands. Not to mention, they won t have very big populations, either, making unit recruitment HARD. Developing these cities will take some time. The barbarian cities are relatively poor and weak. Another perk is that your starting army contains a unit of Roman archers and Triarii! How handy! Disadvantages: Besides, once you gain the upper hand, barbarians flee like the uncivilized savages they are. The barbarians themselves cannot make siege equipment (although there may be a mod (is there?), which, when enabled, will allow barbarians to recruit ballistae and onagers from captured artillery ranges). True, the barbarians field powerful early-game and late-game units such as Berserkers, Foresters and British Chariots, but with the right strategy, combined with the hardy Roman troops, a beginner can deal with these problems. Most of the barbarian armies are not as good compared to the civilized, disciplined legions of Rome. Short campaign goals: Hold 15 provinces and outlast or destroy the Gauls ![]() ![]() Long campaign goals: Hold 50 provinces including Rome The noble Julii have been tasked with exterminating the unwashed barbarians of Gaul, Britannia, Spain and Germania. This is all outlined in greater detail below. This is indeed a very tough choice, as the three Roman campaigns and families have their disadvantages and advantages. The three Roman factions that are playable at the beginning of the game (before you have unlocked any other factions) are the Julii, the Scipii and the Brutii.Ī common question that plagues the new player is: Which faction do I want to play? ![]() |